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Table 1 Anti-VEGF antibody recovered mass in aflibercept and ziv-aflibercept subjected to different storage conditions

From: Evaluation of aflibercept and ziv-aflibercept binding affinity to vascular endothelial growth factor, stability and sterility after compounding

 

Anti-VEGF recovered mass (mg)

Aflibercept

Ziv-aflibercept

Expected mass

2.05

1.28

Pre-assay

2.03 ± 0.02

1.33 ± 0.01

14 days/4 °C

2.08 ± 0.10

1.17 ± 0.14

14 days/− 8 °C

2.21 ± 0.02

1.26 ± 0.11

28 days/4 °C

2.09 ± 0.17

1.28 ± 0.00

28 days/− 8 °C

1.97 ± 0.10

1.25 ± 0.06

  1. Different samples of aflibercept and ziv-aflibercept were added to ELISA plates and detected by HRP-conjugated anti-human IgG and H2O2-OPD substrate. Anti-VEGF quantification (mg) was based on a standard curve performed with both drugs. The drugs were initially diluted in saline (1:256,000 for aflibercept; 1:128,000 for ziv-aflibercept). Statistical analysis was performed comparing the samples to the moment condition of each drug. Representative result of three experiments (mean)
  2. The data are expressed as the mean ± SD
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International Journal of Retina and Vitreous

ISSN: 2056-9920

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