Table 1 Characterization of PRO-169
Test | Method | PRO-169 |
|---|---|---|
Quality | Appearance | Slightly yellow, opalescent liquid |
pH | 6.2 | |
Charge of heterogeneity | CEX-HPLC | 55.1% main peak, 34.3% acidic variants, and 10.7 basic variants |
Structure | Intact mass LC/MS, G0F/G0F | 149201 Da |
Deglycosylated partially reduced LC/MS | Light chain mass = 23450.8 Da, deglycosylated heavy chain mass = 49717.5 Da | |
Oligosaccharide profile | N-Glycan profiling | 79.2% G0, 16.1% G1, and 0.9% G2 |
Other | SPR | KD = 6.54 × 10−6 |
Vitreous Pharmacokinetics | SPR | CMax ± SD = 593.7 ± 42.6 µg/ml |
TMax ± SD = 0.53 ± 0.8 days | ||
T1/2 ± SD = 4.99 ± 0.9 days |