Fig. 2
From: Successful growth of fresh retinoblastoma cells in chorioallantoic membrane
Representative photographs of CAM assay as described. Negative control (PBS) showing the normal vasculature and clear aspect of CAM, positive control (Retinoblastoma) with the development of a white tumor consistent with retinoblastoma (outlined by dotted circle). For the treated samples with different concentrations of Melphalan (20 to 50 µg): Melphalan 20 and 30 µg images show a pronounced tear in the CAM (arrowhead) caused by the accelerated growth of retinoblastoma cells; Melphalan 40 and 50 µg images show hemorrhages (white asterisks), thrombosis and fibrosis (arrows) but with reduced tumor growth