Fig. 3

Affymetrix Oncoscan array performed from tumor-free paraffin-embedded retina and also from fixed retinoblastoma sample. The results have been analyzed with the Chromosome Analysis Suite software, applying the following filters in the analysis: at least 500 altered markers at 500 kb for CNV and at least 1 marker altered in 20 mb for LOH. The genome version was Hg19. Oncoscan by Affymetrix does not allow calculating the mosaicism percentage; therefore, the figures obtained are an approximation. A Affymetrix Oncoscan array from tumor-free paraffin-embedded healthy retina. It carries the 13q deletion in mosaicism but in about 50% of studied cells. B Affymetrix Oncoscan array from fixed retinoblastoma sample. 13q deletion is detected with a frequency consistent with heterozygosity in all tumor cells. Neither LOH nor chromothripsis in 13q bands were detected. 6p12.3pter gain and 6q25.3qter loss were detected as well