Fig. 5

ApoE-rich Deposits That Form Below Cultured RPE Cells Activate the Classical Complement Pathway:Primary human RPE cells grown on porous supports produce globular extracellular deposits. Cultures were exposed to normal or complement-depleted human serum and then processed for immunohistochemistry with various antibody markers. A. Anti-ApoE with no serum exposure; B. Anti-C1q after exposure to normal human serum; C. Anti-C5b-9 after exposure to normal human serum; D. Anti-C5b-9 after expsoure to C1q-depleted human serum. Depletion of factor B had no impact on C5b-9 staining, implicating C1q-dependent/classical cascade activation [96]