Fig. 1From: In vitro differentiation of cGMP-grade retinal pigmented epithelium from human embryonic stem cellsWA09 human embryonic stem cells (hESCs) cultured at the Cell and Molecular Therapy Center. a The colonies grown over Matrigel have a homogeneous shape, smooth and regular edges, and no regions with altered color. b WA09 hESCs have a high ratio of nucleus to cytoplasm. c The colonies are passaged manually by mechanical fragmentationBack to article page