Fig. 3
From: In vitro differentiation of cGMP-grade retinal pigmented epithelium from human embryonic stem cells
Enrichment for human embryonic stem cell-retinal pigment epithelium (hESC-RPE) cells. When the pigmented regions stopped expanding, the cultures were enriched. a The unpigmented areas are scraped off the plate and the remaining cells are passaged enzymatically to a Synthemax-treated well. b After up to three rounds of enrichment, the hESC-RPE cultures are homogenous, with purity exceeding 99%. c The typical high degree of pigmentation and cobblestone morphology of a hESC-RPE homogenous culture