Fig. 7
From: In vitro differentiation of cGMP-grade retinal pigmented epithelium from human embryonic stem cells
Flow cytometric analysis of human embryonic stem cell-retinal pigment epithelium (hESC-RPE) cells. Samples of differentiated hESC-RPE cells at passage 3 are collected, fixed, incubated in the presence of anti-Octamer-binding transcriptions factor 4 (OCT4) antibody, and analyzed. After 120 days of differentiation plus 90 days of enrichment, less than 2% of the cells are positive for OCT4. a The plot shows hESC-RPE cell unstained dispersion (control), b The plot shows hESC-RPE cells and anti-OCT4 dispersion. c The histogram shows the overlaying of a, b