Table 1 Sequence of primers used to monitor retinal pigment epithelial differentiation
From: In vitro differentiation of cGMP-grade retinal pigmented epithelium from human embryonic stem cells
Primer | Sequence |
|---|---|
hBEST1-RT-F | GCCTGCTGAACGAGATGAACA |
hBEST1-RT-R | GCTGTACACCGCCACAGTCA |
hCRALBP-RT-F | CGAGTGGTCATGCTCTTCAACA |
hCRALBP-RT-R | CTGCAAGATCTCATCAAAGGTGA |
hEZRIN-RT-F | AATGCCGAAACCAATCAATGT |
hEZRIN-RT-R | AGTTGTATTTGGCTGGATTGCA |
hHMBS-RT-F | TGGACCTGGTTGTTCACTCCTT |
hHMBS-RT-R | CAACAGCATCATGAGGGTTTTC |
hPAX6-RT-F | CACCGGTTTCCTCCTTCACA |
hPAX6-RT-R | TGGCAGAGCGCTGTAGGTGT |
hRPE65-RT-F | CCTCCTGCACAAGTTTGACTTTAA |
hRPE65-RT-R | GGAAAGCACAGGTGCCAAATT |
hZO1-RT-F | CATCAGATCATTCTGGTCGATCA |
hZO1-RT-R | CCGGAGACTGCCATTGCT |