Table 2 Summary measurements of cytokines and chemokines by evaluation moments and agitation condition in the SR syringe

Cytokines

IL-12p70(pg/mL)

0.668

0.880

0.170

 SP

0.132 (0.279)

0.000 (0.000)

− 0.132 (0.279)

0.057^ǂ

 CP

0.092 (0.237)

0.077 (0.243)

− 0.015 (0.055)

<0.001^ǂ

TNF (pg/mL)

0.995

0.023

0.579

 SP

0.368 (0.581)

0.064 (0.138)

− 0.304 (0.579)

0.044^ǂ

CP

0.369 (0.264)

0.169 (0.303)

− 0.200 (0.235)

0.263^ǂ

IL-10 (pg/mL)

0.631

0.969

0.872

 SP

0.143 (0.226)

0.166 (0.254)

0.023 (0.330)

1.000^ǂ

 CP

0.209 (0.475)

0.211 (0.281)

0.002 (0.283)

1.000^ǂ

IL-6 (pg/mL)

0.417

0.013

0.085

 SP

25,815 (42,437)

936,687 (1.412,46)

910.872 (1.409,668)

1,000^ǂ

 CP

2.120,673 (6,649,132)

7.742,183 (10,103,569)

5.621,51 (9,013,125)

0.007^ǂ

IL-1β (pg/mL)

0.346

0.330

0.903

 SP

0.174 (0.291)

0.069 (0.155)

− 0.105 (0.344)

0.512^ǂ

 CP

0.089 (0.268)

0.000 (0.000)

− 0.089 (0.268)

0.671^ǂ

IL-8 (pg/mL)

0.891

0.068

0.651

 SP

84,172 (124,376)

149,36 (168,312)

65,188 (68,703)

0.396^ǂ

 CP

93,253 (162,579)

190,866 (163,324)

97,613 (228,698)

0.108^ǂ

Chemokines

IP-10 (pg/mL)

0.593

0.005

0.057

 SP

112,071 (77,715)

146,979 (99,204)

34,908 (84,366)

1.000^ǂ

 CP

176,633 (316,682)

390,071 (457,088)

213,438 (300,935)

0,003^ǂ

MCP-1 (pg/mL)

0.861

0.080

0.213

 SP

1.023,24 (683,41)

2.243,311 (1.477,744)

1.220,071 (918,231)

1.000^ǂ

 CP

1.508,242 (2.320,969)

7.588,443 (12.720,342)

6.080,201 (12.976,316)

0.055^ǂ

MIG (pg/mL)

0.530

0.138

0.148

 SP

133,928 (231,658)

129,173 (202,629)

− 4.755 (47,310)

1.000^ǂ

 CP

61,951 (88,722)

235,858 (434,639)

173,907 (409,053)

0.093^ǂ

RANTES (pg/mL)

0.048

0.653

0.360

 SP

0.548 (0,858)

0.837 (0,629)

0.289 (0,818)

1.000^ǂ

 CP

1.936 (2,905)

1.552 (1,178)

− 0.384 (2,312)

0.921^ǂ

IL-8 (pg/mL)

0.568

<0.001

0.058

 SP

77,427 (87,428)

134,79 (143,603)

57,363 (89,646)

0.139^ǂ

 CP

49,779 (57,029)

191,932 (143,606)

142,153 (118,93)

<0.001^ǂ